Hyphal cells can form thick mats and clog the microfluidic system within a cytometer. Filamentous species of fungi contain elongated hyphal cells with nuclei separated by septa. While different ploidy levels are common in fungi, cell morphology changes can interfere with ploidy analysis. However, factors that interfere with cell separation lead to skewed results that are not representative of the true single cell shape, size, or cellular content. The power of flow cytometry lies within its ability to separate single cells from within a population and generate a numerical data output. These lasers generate data characterizing cell size, membrane complexity, and abundance of internal fluorescent markers/dyes and external fluorescent components of the membrane. Hydrodynamic focusing of cells within a stream of liquid allows for single cell interrogation by lasers of different wavelengths. Flow cytometry is the analysis of single cells in suspension.
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